![tracking in fast-forward any-maze tracking in fast-forward any-maze](https://i.ytimg.com/vi/nvlvbAU7sZA/hqdefault.jpg)
Wait until the ene of the tracking process recording or press the stop button on the time control panel. The blinded observers are separated by a curtain. In the testing room collect the EPM data in two ways, manually and by using the software. Then, start the data acquisition process by pressing the start button on the time control panel.
![tracking in fast-forward any-maze tracking in fast-forward any-maze](https://i.ytimg.com/vi/n8rudj4j50Y/mqdefault.jpg)
Place the animal into the experimental area. Finally, set up a new experiment in the movement tracking software system by using the instruction manual. Then, fix the camera above the experimental area, and ensure that it will stay immobile for the duration of the experiment.
Tracking in fast forward any maze install#
Plug the installation key of the movement tracking software into a USB 2.0 port and install the software. Ensure all four arms are similarly illuminated. Light up the EPM Apparatus by using indirect lighting from the ceiling instead of directly illuminating it. Next, familiarize the animals to the intragastric gavage method using water by gavage for five days before ketone supplementation.įor the anxiety assay, use the elevated plus maze or EPM Apparatus, a plus shaped apparatus with four arms with two open and two closed. Then, measure the body weight of the animals before starting any treatments to determine the dosage calculation for treatment. Several days before testing, handle the animals by picking them by their torso and holding them for a minute to acclimate them to the experimenter. Collin Park, a collaborator from my laboratory, will help demonstrating the procedure. The main advantage of this method is that is relies on the rodent's instinctive proclivity to both dark and closed spaces, in addition to the unconditioned fear of heights and avoidance of open spaces. The elevated plus maze is a method to document the effect of various potentially anxiolytic treatments on laboratory rodent models in a more humane way compared to other methods.